Reproductive fluids, used for the in vitro production of pig embryos, result in healthy offspring and avoid aberrant placental expression of PEG3 and LUM

Abstract Background In vitro embryo production (IVP) and transfer (ET) are two very common assisted reproductive technologies (ART) in human cattle. However, pig, the combination of either procedures, or even their use separately, is still considered suboptimal due to low efficiency IVP plus difficulty performing ET long contorted uterus sow. addition, potential impact these ART on health offspring unknown. We investigated here if a modified system, with natural fluids (RF) as supplements culture media, combined minimally invasive surgery perform ET, affects output own system well performance mother placental molecular traits. Results The blastocyst rates obtained by both systems, conventional (C-IVP) (RF-IVP), were similar. Pregnancy farrowing also when compared vivo control (artificial insemination, AI), litter sizes groups lower, while was higher AI than RF-IVP. Gene expression studies revealed aberrant levels for PEG3 LUM tissue C-IVP group AI, but not RF-IVP group. Conclusions additives media pig does improve recipient mothers could mitigate artificial procedures offspring.